The clock starts at 6:00, which is where daytime starts. It is considered to be day between 6:00 to 18:00, and night from 18:00 to 6:00. Each complete cycle takes 12 minutes (6 minutes day and 6 minutes night). There are only 2 ways of interrupting this cycle, and it is with Night Stalker's ultimate, Darkness and Luna's ultimate, Eclipse. While under the effect of it the clock will be paused.

Trees

All trees (except those summoned by Furion's Sprout) will respawn every 5 minutes, starting when the first lane creeps spawn. Trees will not spawn where there is a hero nearby, this so heroes aren't as easily trapped.